Journal
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
Volume 79, Issue 2, Pages 347-351Publisher
WILEY
DOI: 10.1002/prot.22906
Keywords
in-cell NMR; protein expression; protein leakage
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Funding
- NSF [MCB-0516547]
- NIH [DP1OD783]
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In-cell nuclear magnetic resonance spectroscopy is a tool for studying proteins under physiologically relevant conditions. In some instances, however, protein signals from leaked protein are observed in the liquid surrounding the cells. Here, we examine the expression of four proteins in Escherichia coli. We describe the controls that should be used for in-cell NMR experiments and show that leakage is likely when the protein being studied exceeds similar to 20% of the total cellular protein. Proteins 2011; 79:347-351. (C) 2010 Wiley-Liss, Inc.
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