Journal
PROTEIN SCIENCE
Volume 22, Issue 11, Pages 1542-1551Publisher
WILEY
DOI: 10.1002/pro.2340
Keywords
antibody; circular dichroism; differential scanning calorimetry; differential scanning fluoroscopy; subclass change; thermodynamic stability
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Funding
- Japanese Society of the Promotion of Science
- World-Leading Innovative R&D on Science and Technology (FIRST Program) from Council for Science and Technology Policy (CSTP)
- Grants-in-Aid for Scientific Research [24000011] Funding Source: KAKEN
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To address how changes in the subclass of antibody molecules affect their thermodynamic stability, we prepared three types of four monoclonal antibody molecules (chimeric, humanized, and human) and analyzed their structural stability under thermal stress by using size-exclusion chromatography, differential scanning calorimetry (DSC), circular dichroism (CD), and differential scanning fluoroscopy (DSF) with SYPRO Orange as a dye probe. All four molecules showed the same trend in change of structural stability; the order of the total amount of aggregates was IgG1
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