Journal
PROTEIN SCIENCE
Volume 19, Issue 3, Pages 372-382Publisher
WILEY
DOI: 10.1002/pro.340
Keywords
proline; hypoprolinemia; glutamate kinase; gamma-glutamyl phosphate reductase; p53; P5CS deficiency
Categories
Funding
- CIPF
- CIBERER
- MEC (Spanish Ministry of Education and Science) [BFU2007-66781]
- Generalitat Valenciana [ACOMP09/051]
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Pyrroline-5-carboxylate synthase (P5CS) is a bifunctional enzyme that exhibits glutamate kinase (GK) and gamma-glutamyl phosphate reductase (GPR) activities. The enzyme is highly relevant in humans because it belongs to a combined route for the interconversion of glutamate, ornithine and proline. The deficiency of Ill activity in humans is associated with a rare, inherited metabolic disease. It is well established that some bacteria and plants accumulate proline in response to osmotic stress. The alignment of P5CSs from different species and analysis of the solved structures of GK and GPR reveal high sequence and structural conservation. The information acquired from different mutant enzymes with increased osmotolerant properties, together with the position of the insertion found in the longer human isoform, permit the delimitation of the regulatory site of GK and P5CS and the proposal of a model of P5CS architecture. Additionally, the GK moiety of the human enzyme has been modeled and the known clinical mutations and polymorphisms have been mapped.
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