Journal
PROTEIN EXPRESSION AND PURIFICATION
Volume 81, Issue 2, Pages 157-165Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2011.09.010
Keywords
Pseudomonas fluorescens; Recombinant; Protein
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Funding
- NIAID NIH HHS [AI-N01-054210] Funding Source: Medline
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A bottleneck to product development can be reliable expression of active target protein. A wide array of recombinant proteins in development, including an ever growing number of non-natural proteins, is being expressed in a variety of expression systems. A Pseudomonas fluorescens expression platform has been developed specifically for recombinant protein production. The development of an integrated molecular toolbox of expression elements and host strains, along with automation of strain screening is described. Examples of strain screening and scale-up experiments show rapid development of expression strains producing a wide variety of proteins in a soluble active form. (C) 2011 Elsevier Inc. All rights reserved.
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