Journal
PROTEIN EXPRESSION AND PURIFICATION
Volume 81, Issue 1, Pages 115-118Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2011.09.011
Keywords
Human respiratory syncytial virus; Fusion glycoprotein; Chromatographic purification
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Funding
- National Natural Science Foundation of China [30972604]
- Beijing Municipal Natural Science Foundation [7092053]
- National Key Technology RD Program [2008BAK41B02-4]
- Fundamental Research Funds for the Central Universities [2011JBM132]
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Human respiratory syncytial virus (RSV) fusion glycoprotein (F) elicits neutralizing antibodies to RSV and has therefore attracted much attention as a suitable candidate antigen in the development of gene-based vaccines against RSV infections. However, a major obstacle in vaccine development has been the problem of antigen purification. To address this problem, we have developed a new method that combines sucrose gradient ultracentrifugation and a two-step chromatographic process, to purify RSV F from RSV particles propagated in HEp-2 cells. Analysis of the fractions produced using this method showed recovery of a functional homodimer with a molecular weight of 140 kDa, and 54% preservation of the original F. (C) 2011 Elsevier Inc. All rights reserved.
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