Journal
PROTEIN EXPRESSION AND PURIFICATION
Volume 80, Issue 2, Pages 283-293Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2011.08.005
Keywords
Carboxypeptidase; Aminopeptidase; PreScission protease; Affinity tag; Polyhistidine tag; His-tag; TEV protease; Enterokinase; Enteropeptidase; Thrombin; 3C protease; Factor Xa
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Funding
- NIH, National Cancer Institute, Center for Cancer Research
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Although they are often exploited to facilitate the expression and purification of recombinant proteins, every affinity tag, whether large or small, has the potential to interfere with the structure and function of its fusion partner. For this reason, reliable methods for removing affinity tags are needed. Only enzymes have the requisite specificity to be generally useful reagents for this purpose. In this review, the advantages and disadvantages of some commonly used endo- and exoproteases are discussed in light of the latest information. Published by Elsevier Inc.
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