Journal
PROTEIN EXPRESSION AND PURIFICATION
Volume 69, Issue 2, Pages 198-203Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2009.08.013
Keywords
Lumbrokinase; Thrombosis model; Escherichia coli; Inclusion body
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Lumbrokinase (LK) is an important fibrinolytic enzyme derived from earthworms. it has been found that LK is composed of a group of isoenzymes. To construct and express the mature peptide of LK PI239 in Escherichia coli, we amplified and optimized the gene of LK which was then cloned into the prokaryotic expression vector pET-22b(-). The recombinant LK (rLK) protein was expressed as inclusion bodies and we have developed a purification process of rLK from these inclusion bodies. A step-down urea concentration strategy was applied to the rLK renaturation process. The purified and renatured rLK apparently ameliorated the conditions of the model thrombosis rats used, and may be developed into a therapeutic agent for thrombotic-associated diseases. (C) 2009 Elsevier Inc. All rights reserved.
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