Journal
PROTEIN EXPRESSION AND PURIFICATION
Volume 72, Issue 2, Pages 149-156Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2010.04.009
Keywords
Tandem affinity purification; Protein-protein interaction; Protein complex; TAP tag
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Funding
- National Outstanding Youth Foundation of China [30625008]
- major project of cultivating new varieties of Transgenic organisms [2009ZX08009-029B]
- National High Technology Research and Development Program (863 Program) [2007AA021401]
- National Basic Research Program of China (973 Program) [2007CB108902]
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Isolation and identification of protein partners in multi-protein complexes are important in gaining further insights into the cellular roles of proteins and determining the possible mechanisms by which proteins have an effect in the molecular environment. The tandem affinity purification (TAP) method was originally developed in yeast for the purification of protein complexes and identification of protein-protein interactions. With modifications to this method and many variations in the original tag made over the past few years, the TAP system could be applied in mammalian, plant, bacteria and other systems for protein complex analysis. In this review, we describe the application of the TAP method in various organisms, the modification in the tag, the disadvantages, the developments and the future prospects of the TAP method. (C) 2010 Elsevier Inc. All rights reserved.
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