Journal
PROTEIN EXPRESSION AND PURIFICATION
Volume 65, Issue 2, Pages 261-266Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2009.01.008
Keywords
Rhizopus oryzae; Glucoamylase; Starch-binding domain; Enhanced green fluorescent protein; Protein purification; Carbohydrate-binding module
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Funding
- Simpson Biotech Co., Ltd., Taiwan, RCC
- National Science Council, ROC [97-2622-B-007-001]
- Council of Agriculture and the National Science and Technology Program for Agricultural Biotechnology [97AS-1.2.1.ST-a5]
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The use of protein fusion tag technology simplifies and facilitates purification of recombinant proteins. In this article, we have found that the starch-binding domain derived from Rhizopus oryzae glucoamylase (RoSBD), a member of carbohydrate-binding module family 21 (CBM21) with raw starch-binding activity, is favorable to be applied as an affinity tag for fusion protein engineering and purification in Escherichia coli and Pichia pastoris systems. To determine suitable spatial arrangement of RoSBD as a fusion handle, enhanced green fluorescent protein (eGFP) was fused to either the N- or C-terminus of the SBD, expressed by E. coli, and purified for yield assessment and functional analysis. Binding assays showed that the ligand-binding capacity was fully retained when the RoSBD was engineered at either the N-terminal or Similar results have been obtained with the RoSBD-conjugated phytase secreted by the C-terminal end. A pastoris. The effective adsorption onto raw starch and low cost of starch make RoSBD practically applicable in terms of development of a new affinity fusion tag for recombinant protein engineering in an economic manner. (C) 2009 Elsevier Inc. All rights reserved.
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