Journal
PROTEIN EXPRESSION AND PURIFICATION
Volume 64, Issue 2, Pages 194-197Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2008.11.002
Keywords
Pichia pastoris; Endothelial cell protein C receptor; Recombinant protein; Surface plasmon resonance; Protein C; Factor VII
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Funding
- Instituto de Salud Carlos III [PI051178]
- Education Department, Gobierno de Navarra
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Endothelial cell protein C receptor (EPCR) downregulates the coagulation system and prevents thrombosis by binding to protein C/activated protein C (APC) and factor VII/activated factor VII (VIIa). Recombinant APC and factor Vila have been shown to be useful in a variety of clinical conditions. Murine models could prove extremely helpful in order to study in vivo actions of these drugs. It is therefore crucial to demonstrate the interaction between these and murine EPCR. We expressed the extracellular region of the murine EPCR in a yeast expression system and obtained a colony of Pichia pastoris that produce high amounts of recombinant extracellular murine EPCR, which we purified by liquid chromatography to homogeneity. The analysis of the interaction of EPCR with APC and factor Vila was carried out using surface plasmon resonance technology. Murine EPCR binds to APC and factor Vila with similar affinity than human EPCR. As for human EPCR, the binding is Ca2+ dependent while Mg2+ ions optimize it. In conclusion, we succeeded in establishing a system to produce enough recombinant soluble murine EPCR to perform biochemical studies. Murine EPCR binds to human APC and factor Vila, which opens up new possibilities for characterizing the in vivo effect of APC and factor VII by using murine models. (C) 2008 Elsevier Inc. All rights reserved.
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