4.1 Article

Functional effects of active site mutations in NAD+-dependent formate dehydrogenases on transformation of hydrogen carbonate to formate

Journal

PROTEIN ENGINEERING DESIGN & SELECTION
Volume 31, Issue 9, Pages 327-335

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/protein/gzy027

Keywords

active site mutations; catalytic functioning; molecular modelling; NAD(+)-dependent formate dehydrogenase; reduction of hydrogen carbonate

Funding

  1. Scientific and Technological Research Council of Turkey-TUBITAK [214Z292]
  2. Gebze Technical University-Scientific Research Projects [2017-A105-62]

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Conversion of hydrogen carbonate to formate by mutants of Candida methylica (CmFDH) and Chaetomium thermophilum (CtFDH) formate dehydrogenases (FDHs) was studied. Hydrogen carbonate is not the primary substrate for the hydride transfer reaction in FDHs. The chosen mutations were selected so that enzyme activity could remain at an adequate level. In CtFDH, the mutation Asn120Cys in the active site inactivated the enzyme for formate (oxidation) but increased the specific activity for hydrogen carbonate (reduction) as a function of substrate concentration. The mutation Asn120Cys in CtFDH increased 6.5-fold the K-M, indicating that substrate binding was weakened. A 6.5-fold increase of k(cat) compensated the lower affinity suggesting that product release was improved. The corresponding mutation Asn119Cys in CmFDH inactivated the enzyme for both substrates. Molecular dynamics simulations indicated that the active site dimensions change differently with different substrates after mutations, and in the mutant Asn120Cys of CtFDH, hydrogen carbonate adopted better reactive position than formate. With hydrogen carbonate, the active site enlarged enough for two hydrogen carbonate molecules to be placed there. The change of Asn119 to bulky Tyr or His in CmFDH requires changes in the active site to accommodate the substrate; activity with formate was retained but not with hydrogen carbonate. This study showed that the active site of FDHs can be modified radically, which gives possibilities for further enzyme engineering to improve the reaction with hydrogen carbonate or carbon dioxide for enzymatic fixing of carbon dioxide.

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