Journal
PROTEIN ENGINEERING DESIGN & SELECTION
Volume 26, Issue 10, Pages 671-674Publisher
OXFORD UNIV PRESS
DOI: 10.1093/protein/gzt019
Keywords
antibodies; human antibody variable domains; phage display; protein aggregation
Funding
- National Health and Medical Research Council
- Australian Research Council
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Aggregation limits the recombinant production of many commercially important proteins. We have recently identified mutations that control the aggregation behavior of human antibody variable domains (Dudgeon K., Rouet R., Kokmeijer I., Schofield P., Stolp J., Langley D., Stock D. and Christ D. (2012) Proc Natl Acad Sci USA, 109, 1087910884. This has allowed the generation of a panel of human antibody variable heavy domains with a defined range of aggregation propensities. Here we utilize this unique resource to validate a previously reported heat-denaturation method on phage (Jespers L., Schon O., Famm K. and Winter G. (2004) Nat Biotechnol, 22, 11611165. Our experiments revealed that the method is not only robust in respect to denaturation conditions on phage, but also highly indicative of solution behavior. In particular, it is an excellent predictor of expression and refolding yields.
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