Journal
PROTEIN ENGINEERING DESIGN & SELECTION
Volume 25, Issue 6, Pages 295-305Publisher
OXFORD UNIV PRESS
DOI: 10.1093/protein/gzs018
Keywords
cardiac troponin I; high-throughput screening; phage display; recombinant antibody; surface plasmon resonance
Funding
- Science Foundation Ireland under CSET [05/CE3/B754, 10/CE/B1821]
- Irish Research Council for Science, Engineering and Technology (IRCSET)
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In vitro diagnostic (IVD) platforms provide rapid and accurate determination of disease status. The clinical performance of antibody-based diagnostic platforms is paramount as the information provided often informs the medical intervention taken and, ultimately, the patients outcome. Breaking down such an immuno-IVD device into its component elements, the biorecognition entity is key to the analytical specificity of the test. Furthermore, tailored optimisation of the antibody is often necessary to impart the desired biophysical properties for the specific application. This tailoring is now widely facilitated by advances in combinatorial approaches to antibody generation, molecular evolution strategies and the availability of truly high-throughput (HT), refined surface plasmon resonance-based screening tools. In this paper, we demonstrate a rational, knowledge-driven approach to the generation of epitope-specific antibodies for the early detection of cardiovascular disease, discuss the merits of the approaches taken and offer a perspective on HT strategies to mining large antibody libraries. These results highlight the expedience of such methodologies for the development of truly superior cardiovascular disease biorecognition elements.
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