Journal
PROTEIN AND PEPTIDE LETTERS
Volume 20, Issue 12, Pages 1373-1381Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/092986652012131112130322
Keywords
Expression systems; large-scale production; mammalian cell culture; protein glycosylation; human cell lines; recombinant proteins
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Funding
- FAPESP [2012/04629-8, 2012/02109-7]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/04629-8, 12/02109-7] Funding Source: FAPESP
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Over the past 20 years the demand for recombinant proteins has increased significantly. Mammalian cell lines have been extensively used to produce recombinant proteins. This expression system offers several advantages over microbial systems, mammalian cells have the cellular machinery to promote the secretion of the recombinant product and the posttranslational modifications, like glycosylation that is present in many of recombinant therapeutic proteins in the market. Human cell lines have emerged as a new and powerful alternative for production of such products. These cells are able to produce recombinant proteins with posttranslational modifications more similar to their natural counterparts, producing proteins with human-like glycosylation pattern avoiding immunogenic reactions against epitopes nonhumans. This review presents the available human cell lines that can be used in pharmaceutical industry, the advantages of this expression system and the main efforts made in this field.
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