Journal
PROTEIN AND PEPTIDE LETTERS
Volume 15, Issue 10, Pages 1075-1078Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/092986608786071058
Keywords
GroEL/ES; Soluble expression; western blotting; normal and variant rice catalase-B; Plant protein; Heterologous expression
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Funding
- JRF
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Expression of plant proteins in E. coli is frequently unsuccessful, but soluble and functional rice catalase-B can be produced in E. coli when it is co-expressed with the chaperone GroEL/ES. The rice catalase exhibited properties typical for a catalase including the decomposition of H2O2 and inhibition by aminotriazole, a specific inhibitor for plant and animal catalases. This achievement records for first time the successful expression of a both native and variant rice plant catalase in bacterial cytosol suggesting that it may be an option to be considered for the expression of other plant proteins in E. coli.
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