4.2 Article

Aptabodies - New type of artificial receptors for detection proteins

Journal

PROTEIN AND PEPTIDE LETTERS
Volume 15, Issue 8, Pages 799-805

Publisher

BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/092986608785203656

Keywords

DNA aptamers; hybridization; human thrombin; multiwalled carbon nanotubes; biosensor; electrochemical quartz crystal microbalance

Funding

  1. European Commission [DVT-IMP, IST-034256]
  2. Slovak Grant Agency [1/4016/07]
  3. Russian Foundation for Basic Research [06-03-32217]

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We report on a new type of artificial receptor formed by hybridization of two DNA aptamers for human thrombin (aptabody). This aptasensor based on multiwalled carbon nanotubes allowed us to detect thrombin with detection limit of 0.3 nM, which was 3 times better in comparison with conventional aptamer.

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