Journal
PROTEIN AND PEPTIDE LETTERS
Volume 15, Issue 8, Pages 799-805Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/092986608785203656
Keywords
DNA aptamers; hybridization; human thrombin; multiwalled carbon nanotubes; biosensor; electrochemical quartz crystal microbalance
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Funding
- European Commission [DVT-IMP, IST-034256]
- Slovak Grant Agency [1/4016/07]
- Russian Foundation for Basic Research [06-03-32217]
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We report on a new type of artificial receptor formed by hybridization of two DNA aptamers for human thrombin (aptabody). This aptasensor based on multiwalled carbon nanotubes allowed us to detect thrombin with detection limit of 0.3 nM, which was 3 times better in comparison with conventional aptamer.
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