Journal
PROSTATE CANCER AND PROSTATIC DISEASES
Volume 13, Issue 4, Pages 356-361Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/pcan.2010.32
Keywords
androgen; LNCaP cells; microRNA; miR-148a; CAND1
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Funding
- MEXT
- NIBIO
- Japan Society for the Promotion of Science
- Ministry of Health, Labor and Welfare
- Promotion and Mutual Aid Corporation for Private School of Japan
- Grants-in-Aid for Scientific Research [21591170] Funding Source: KAKEN
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Recent advances in cancer biology reveal that microRNAs (miRNAs) are involved in the regulation of cancer-related genes, or they function as tumor suppressors or oncogenes. In prostate cancer, evidence has accumulated for the contribution of the androgen-dependent gene network to tumor growth, although the precise functions of miRNAs in prostate cancer remain to be investigated. Here, we identified androgen-responsive miRNAs by the short RNA sequencing analysis in LNCaP prostate cancer cells. Among 10 miRNAs with known sequences, we have determined that miR-148a reduces the expression of cullin-associated and neddylation-dissociated 1 (CAND1), a negative regulator of SKP1-Cullin1-F-box (SCF) ubiquitin ligases, by binding to the 3'-untranslated region of CAND1 mRNA. CAND1 knockdown by small interfering RNA promoted the proliferation of LNCaP cells. Our study indicates the potential contribution of miR-148a to the growth of human prostate cancer. Prostate Cancer and Prostatic Diseases (2010) 13, 356-361; doi: 10.1038/pcan.2010.32; published online 7 September 2010
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