Journal
PROSTATE CANCER AND PROSTATIC DISEASES
Volume 13, Issue 2, Pages 195-201Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/pcan.2009.55
Keywords
TRPM2; siRNA; PARP; proliferation
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Funding
- ADA [DOD PA031119]
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We have identified a novel function for a member of the transient receptor potential (TRP) protein super-family, TRPM2, in prostate cancer cell proliferation. TRPM2 encodes a non-selective cation-permeable ion channel. We found that selectively knocking down TRPM2 with the small interfering RNA technique inhibited the growth of prostate cancer cells but not of non-cancerous cells. The subcellular localization of this protein is also remarkably different between cancerous and non-cancerous cells. In BPH-1 (benign), TRPM2 protein is homogenously located near the plasma membrane and in the cytoplasm, whereas in the cancerous cells (PC-3 and DU-145), a significant amount of the TRPM2 protein is located in the nuclei in a clustered pattern. Furthermore, we have found that TRPM2 inhibited nuclear ADP-ribosylation in prostate cancer cells. However, TRPM2 knockdown-induced inhibition of proliferation is independent of the activity of poly(ADP-ribose) polymerases. We conclude that TRPM2 is essential for prostate cancer cell proliferation and may be a potential target for the selective treatment of prostate cancer. Prostate Cancer and Prostatic Diseases (2010) 13, 195-201; doi: 10.1038/pcan.2009.55; published online 22 December 2009
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