Journal
PROSTATE
Volume 71, Issue 16, Pages 1736-1745Publisher
WILEY
DOI: 10.1002/pros.21390
Keywords
prostate cancer; urine; biomarkers; PSA; PSMA; PSGR; PCA3
Categories
Funding
- Instituto de Salud Carlos III, Ministerio de Ciencia e Innovacion [RTICC RD06/0020/0058, CP10/00355, PS 09-00496]
- Red de Genomica del Cancer y Genotipado de tumores [C03/10]
- Fundacion para la Investigacion en Urologia [FIU-2007]
- Departament d'Univeristats, Recerca I Societat de la Informacio de la Generalitat de Catalunya [SGR00487, 2009-TEM 00066]
- Fundacion Francisco Cobos [Jovenes Licenciados 2008]
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BACKGROUND. Several studies have demonstrated the usefulness of monitoring an RNA transcript, such as PCA3, in post-prostate massage (PM) urine for increasing the specificity of prostate-specific antigen (PSA) in the detection of prostate cancer (PCa). However, a single marker may not necessarily reflect the multifactorial nature of PCa. METHODS. We analyzed post-PM urine samples from 154 consecutive patients, who presented for prostate biopsies because of elevated serum PSA (> 4 ng/ml) and/or abnormal digital rectal exam. We tested whether the putative PCa biomarkers PSMA, PSGR, and PCA3 could be detected by quantitative real-time PCR in post-PM urine sediment. We combined these findings to test if a combination of these biomarkers could improve the specificity of actual diagnosis. Afterwards, we specifically tested our model for clinical usefulness in the PSA diagnostic gray zone (4-10 ng/ml) on a target subset of 82 men with no prior biopsy. RESULTS. By univariate analysis, we found that the PSMA, PSGR, and PCA3 scores were significant predictors of PCa. Using a multiplex model, the area under the multi receiver-operating characteristic curve was 0.74 versus 0.82 in the diagnostic gray zone. Fixing the sensitivity at 96%, we obtained a specificity of 34% and 50% in the gray zone. CONCLUSIONS. Taken together, these results provide a strategy for the development of a more accurate model for PCa diagnosis. In the future, a multiplexed, urine-based diagnostic test for PCa with a higher specificity, but the same sensitivity as the serum-PSA test, could be used to determine better which patients should undergo biopsy. Prostate 71: 1736-1745, 2011. (C) 2011 Wiley Periodicals, Inc.
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