Journal
PROSTAGLANDINS & OTHER LIPID MEDIATORS
Volume 93, Issue 1-2, Pages 52-59Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.prostaglandins.2010.06.005
Keywords
Mesenchymal stem cells; PGF(2 alpha); AKR1B3; Adipogenesis; FP receptor
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [21570151, 22300133]
- Japan Science and Technology Agency
- Suzuken Memorial Foundation
- Sumitomo Foundation
- Japanese Biochemical Society
- Takeda Science Foundation
- Japan Foundation for Applied Enzymology
- Grants-in-Aid for Scientific Research [21570151, 22300133] Funding Source: KAKEN
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In this study, we investigated the roles of prostaglandin (PG) F-2 alpha in the differentiation of mouse ST2 mesenchymal stem cells (MSC) into adipocytes and osteoblasts PGF(2 alpha) was not produced in the undifferentiated ST2 MSC, but its highest level of production was detected at 3 h after the initiation of adipogenesis and then quickly decreased On the contrary, apparent PGF(2 alpha) production was not detected during the osteoblastogenesis of ST2 MSC The PGF(2 alpha) production pattern during adipogenesis well resembled the expression profiles of aldo-keto reductase (AKR) 1B3, which acted as the PGF(2 alpha) synthase, and cyclooxygenase-2 genes, but the pattern showed a slight delay compared with these profiles The siRNA for AKR1B3, but not that for AKR1B8 or 1B10, decreased PGF(2 alpha) production and enhanced the expression of adipogenic genes, but did not affect the m RNA levels of osteoblastogenic genes, during the adipogenesis of ST2 MSC The FP receptor was expressed during adipogenesis of ST2 MSC, and its agonist or antagonist suppressed or enhanced, respectively, the lipid accumulation and the adipogenic gene expression, but this receptor was not associated with the osteoblastogenesis These results indicate that AKR1B3-mediated PGF(2 alpha) suppressed the early phase of adipogenesis through FP receptors, but did not affect osteoblastogenesis in ST2 MSC Therefore, PGF(2 alpha) suppressed the progression of early phase adipogenesis after determination of the cell fare that causes MSC to differentiate into adipocytes. (C) 2010 Elsevier Inc All rights reserved
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