4.2 Article

High-dose lovastatin decreased basal prostacyclin production in cultured endothelial cells

Journal

PROSTAGLANDINS & OTHER LIPID MEDIATORS
Volume 89, Issue 1-2, Pages 1-7

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/j.prostaglandins.2008.12.005

Keywords

Lovastatin; 27OHC; PLA(2); Cyclooxygenase-1; Arachidonic acid; Prostacyclin

Funding

  1. Wallace Research Foundation
  2. Verna L. and John R. Hildebrand Foundation

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The effect of lovastatin, a 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, on prostacyclin production was studied in cultured human umbilical vein endothelial cells. The results indicated that lovastatin induced a significant dose- and time-dependent decrease of arachidonic acid release from the cells, an inhibition of phospholipase A(2) (PLA(2)) activity, a reduction of [H-3]choline in lysophosphatidylcholine (lysoPC), and a diminishment of Ca-45(2+) influx into the cells. The decreased arachidonic acid release was not reversed by addition of either intermediate products during cholesterol synthesis, such as mevalonate, geranylgeraniol, farnesol, or cholesterol and lipoprotein although a reduced concentration of cholesterol in the cells, caused by lovastatin, was reversed by added cholesterol. Lovastatin, furthermore, diminished prostacyclin production and inhibited activity of cyclooxygenase-1. 27-Hydroxycholesterol (27OHC), an oxidized cholesterol, had the same effect on HMG-CoA reductase as lovastatin. but 27OHC increased arachidonic acid release and Ca-45(2+) influx. Our results indicated that lovastatin inhibited significantly activities Of PLA(2) and cyclooxygenase-1, resulting in a marked reduction in arachidonic acid release, lysoPC content and prostacyclin production in the cultured vascular endothelial cells. (C) 2009 Published by Elsevier Inc.

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