Journal
NATURE COMMUNICATIONS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms8147
Keywords
-
Categories
Funding
- NIH/NIGMS [RO1GM61936, 5DP1GM106412]
- Harvard Medical School (HMS)
- NIH/NCI [F32CA157188]
- Centre National de la Recherche Scientifique
- Fulbright Visiting Scholar Program
- NIH [1DP2OD007292, 1R01EB018659, 5R21HD072481, 1DP2OD004641, RO1-GM090278]
- HHMI
- Damon Runyon Cancer Research Foundation
- ONR [N000141110914, N000141010827, N000141310593]
- NSF [CCF1054898, CCF1162459]
- Wyss Institute for Biologically Engineering
- Alexander von Humboldt-Foundation
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1317694] Funding Source: National Science Foundation
Ask authors/readers for more resources
Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging. We first describe the in situ visualization of single-copy regions of the genome using two single-molecule super-resolution methodologies. We then introduce a robust and reliable system that harnesses single-nucleotide polymorphisms (SNPs) to visually distinguish the maternal and paternal homologous chromosomes in mammalian and insect systems. Both of these new technologies are enabled by renewable, bioinformatically designed, oligonucleotide-based Oligopaint probes, which we augment with a strategy that uses secondary oligonucleotides (oligos) to produce and enhance fluorescent signals. These advances should substantially expand the capability to query parent-of-origin-specific chromosome positioning and gene expression on a cell-by-cell basis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available