Journal
PROCESS BIOCHEMISTRY
Volume 75, Issue -, Pages 187-193Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2018.09.012
Keywords
Lignin; Pandoraea sp. B-6; THCB pretreatment; THC pretreatment; Corn stover; Enzymatic digestibility
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Funding
- National Natural Science Foundation of China [51634010]
- China Postdoctoral Science Foundation [2017M612594]
- Postdoctoral Science Foundation of Central South University [140050002]
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Chemical pretreatments significantly improve the enzymatic hydrolysis of lignocellulosic biomass, however, lignin, more or less, left in the residue still presents a significant challenge for the sugar yield. To address this challenge, we proposed a pretreatment strategy using bacteria-assisted (tetrahydrofuran) THF-H2O co-solvent (THCB) to enhance enzymatic hydrolysis under mild conditions. Pandoraea sp. B-6 was proved to effectively remove lignin (to 3.7%) in the corn stover (CS) residue after THF-H2O co-solvent (THC) pretreatment. The compositional and structural analysis confirmed that lignin removal contributed to the expose of more cellulose. The sugar yield varied from 129.6 mg/g to 827.2 mg/g after THCB pretreatment under different conditions, which was increased by 42.6% similar to 2.8% over THC pretreatment (90.8 mg/g similar to 804.5 mg/g) and 1.5 similar to 9.7 times over the untreated CS (84.8 mg/g). Our founding provides a promising technology for lignocellulosic biomass pretreatment in terms of advanced lignin removal, enzymatic digestibility, and reduced energy consumption.
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