Journal
PROCESS BIOCHEMISTRY
Volume 49, Issue 12, Pages 2235-2240Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2014.09.015
Keywords
Direct interspecies electron transfer (DIET); Magnetite nanoparticles; Reductive dechlorination, TCE
Categories
Funding
- CNR
- Regione Lombardia
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The interaction of anaerobic dechlorinating cultures with soil and aquifer geochemical components is largely unknown, although this has potentially a major impact on the bioremediation of chlorinated solvent-contaminated sites. In this study, we found that addition of magnetite (Fe3O4) - the end-product of Fe(III)-reduction by dissimilatory iron reducing bacteria - to anaerobic dechlorinating cultures enhances the kinetics of trichloroethene dechlorination up to 1.5-times, compared to unamended controls. Specifically, a low concentration (approx. 10 mg/L as total Fe) of small size particles (200 nm-filtered) resulted in a greater stimulatory effect compared to the addition of a higher concentration (approx. 300 mg/L as total Fe) of unfiltered particles. Notably, Desulforomonas spp. were substantially enriched in microcosms supplemented with magnetite, whereas Dehalococcoides mccartyi spp. was found to be markedly inhibited or outcompeted. Multiple lines of evidence, including the direct visualization of microbial cells and magnetite particles via Confocal Laser Scanning Microscopy (CLSM), suggest that electrically conductive particles promoted the establishment of a cooperative metabolism, based on direct interspecies electron transfer, between dechlorinating and non-dechlorinating microorganisms. (C) 2014 Elsevier Ltd. All rights reserved.
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