Journal
PROCESS BIOCHEMISTRY
Volume 49, Issue 2, Pages 318-323Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2013.12.003
Keywords
Amur sturgeon (Acipenser schrenckii); Collagen; Cartilage; Isolation; Characterization
Categories
Funding
- Scientific Research Starting Foundation for Talented Scholars of Jiangsu University [10JDG053]
- China Postdoctoral Science Foundation [2011M500872]
Ask authors/readers for more resources
The collagen in Amur sturgeon cartilage was isolated using sodium chloride (salt-solubilized collagen, SSC, 2.18%), followed by acetic acid (acid-solubilized collagen, ASC, 27.04%) and then pepsin (pepsinsol ubilized collagen, PSC, 55.92%). These collagens appeared to be dense sheet-like film linked by randomcoiled filaments under SEM. The denaturation and melting temperatures of PSC (35.71 and 123.90 degrees C) were significantly higher than SSC (32.64 and 114.51 degrees C) and ASC (32.98 and 120.72 degrees C) assessed by circular dichroism and differential scanning calorimetry, which could be attributed to its high imino acid content (22.57%) and degree of hydroxylation (47.29%). Electrophoresis pattern showed that the SSC and ASC were type I collagen, while PSC was predominantly type II collagen along with other minor types. Infrared spectra confirmed their triple helical structure, and indicated more hydrogen bonding in ASC and more intermolecular crosslinks in PSC. These results provide some basis for their large-scale production and further application as alternatives to mammalian collagen. (c) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available