Journal
PROCESS BIOCHEMISTRY
Volume 49, Issue 3, Pages 357-364Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2013.12.019
Keywords
Bio-ethanol; Yeast cell-free enzyme system; Glycolytic and fermentation enzymes; SDS-PAGE analysis; Fermentation
Categories
Funding
- National Research Foundation (NRF) of Korea
- Ministry of Education, Science and Technology [2011-0016965]
- National Research Foundation of Korea [2011-0016965] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A yeast cell-free enzyme system containing an intact fermentation assembly and that is capable of bio-ethanol production at elevated temperatures in the absence of living cells was developed to address the limitations associated with conventional fermentation processes. The presence of both yeast glycolytic and fermentation enzymes in the system was verified by SDS-PAGE and LC-MS/MS Q-TOF analyses. Quantitative measurements verified sufficient quantities of the co-factors ATP (1.8 mM) and NAD(+) (0.11 mM) to initiate the fermentation process. Bio-ethanol was produced at a broad temperature range of 30-60 degrees C but was highly specific to a pH range of 6.0-7.0. The final bio-ethanol production at 30, 40, 50, and 60 degrees C was 3.37, 3.83, 1.94, and 1.60 g/L, respectively, when a 1% glucose solution was used, and the yield increased significantly with increasing cell-free enzyme concentrations. A comparative study revealed better results for the conventional fermentation system (4.46 g/L) at 30 degrees C than the cell-free system (3.37 g/L); however, the efficacy of the cell-free system increased with temperature, reaching a maximum (3.83 g/L) at 40 degrees C, at which the conventional system could only produce 0.48 g/L bio-ethanol. Successful bio-ethanol production using a single yeast cell-based enzyme system at higher temperatures will lead to the development of novel strategies for efficient bio-ethanol production through SSF. (C) 2014 Elsevier Ltd. All rights reserved.
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