Journal
PROCESS BIOCHEMISTRY
Volume 48, Issue 5-6, Pages 775-781Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2013.03.003
Keywords
Lignocellulose; Experimental design; Extrusion; Enzymatic hydrolysis; Alkaline treatment
Categories
Funding
- Regional Government of Madrid [P2009/ENE-1743]
- European Community [227498]
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In this work, an integrated one-step alkaline extrusion process was tested as pretreatment for sugar production from barley straw (BS) biomass. The influence of extrusion temperature (T) and the ratio NaOH/BS dry matter (w/w) (R) into the extruder on pretreatment effectiveness was investigated in a twin-screw extruder at bench scale. A 2(3) factorial design of experiments was used to analyze the effect of process conditions [T: 50-100 degrees C; R: 2.5-7.5% (w/w)] on composition and enzymatic digestibility of pretreated substrate (extrudate). The optimum conditions for a maximum glucan to glucose conversion were determined to be R = 6% and T = 68 degrees C. At these conditions, glucan yield reached close to 90% of theoretical, while xylan conversion was 71% of theoretical. These values are 5 and 9 times higher than that of the untreated material, which supports the great potential of this one-step combined pre-treatment technology for sugar production from lignocellulosic substrates. The absence of sugar degradation products is a relevant advantage over other traditional methods for a biomass to ethanol production process since inhibitory effect of such product on sugar fermentation would be prevented. (C) 2013 Published by Elsevier Ltd.
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