Journal
PROCESS BIOCHEMISTRY
Volume 48, Issue 9, Pages 1395-1401Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2013.06.029
Keywords
Marine fungus; Aspergillus terreus; Extracellular polysaccharide; Structural characterization; Antioxidant property
Categories
Funding
- Science and Technology Development Program of Shandong Province, China [2010GHY10509]
- National Oceanographic Center of Qingdao of China
- National Natural Science Foundation of China [41076086]
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The marine fungus Aspergillus terreus produces an extracellular polysaccharide, YSS, when grown in potato dextrose-agar medium. YSS was isolated from the fermented liquids using ethanol precipitation, anion-exchange and size-exclusion chromatography. YSS was mainly composed of mannose and galactose in a molar ratio of 7.68:1.00, its average molecular weight was estimated to be about 18.6 kDa. On the basis of chemical and spectroscopic analyses, including one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) spectroscopy, structure of YSS may be represented, at an average, as a backbone of mannan with two types of branches. The mannan backbone is mainly composed of (1 -> 2)-linked alpha-mannopyranose with small amounts of (1 -> 6)-linked alpha-mannopyranose residues. The branches consist of terminal beta-galactofuranose residues, and disaccharide units of (1 -> 6)-linked alpha-mannopyranose. The branches are linked to C-6 of (1 -> 2)-linked alpha-mannopyranose residues of backbone. The antioxidant activity of YSS was evaluated with the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals in vitro, and the results indicated that YSS had good antioxidant activity, especially scavenging ability on DPPH radicals. The investigation demonstrated that YSS is a novel branched galactomannan with antioxidant activity, and differs from previously described extracellular polysaccharides. (C) 2013 Elsevier Ltd. All rights reserved.
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