Journal
PROCESS BIOCHEMISTRY
Volume 48, Issue 8, Pages 1212-1215Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2013.06.003
Keywords
Lipase 2; Fatty acid ethyl esters (FAEEs); Saccharomyces cerevisiae
Categories
Funding
- National Nature Science Foundation of China [21076017, 20876012]
- Key Project in the National Pillar Program [2011BAD22B04]
- 973 Project [2011CB710800, 2011CB200905, 2009CB724703]
- National 863 High-Tech Project [2009AA03Z232, 2009AA02Z207]
- Nature Science Foundation of Beijing [Z09010300840901]
Ask authors/readers for more resources
In this study, we expressed lipase 2 from Candida sp. 99-125 in Saccharomyces cerevisiae, and tried direct biodiesel production. Driven by 3-phosphoglycerate kinase promoter, Lip2 showed high expression level in cytoplasm. SDS-PAGE analysis confirmed the successful lipase expression with a 40 kDa molecular weight. The enzyme assay indicated that lipase 2 had a specific activity of 12.12 mu mol/min/mg toward p-nitrophenyl palmitate. Gas chromatography showed that the main fatty acids of S. cerevisiae lipids were palmitoleic acid (31.79%) and oleic acid (29.84%). By three-step addition of 4% ethanol to culture broth, the yield of fatty acid ethyl esters by recombinant S. cerevisiae reached 11.4 mg/g dry cell weight. This work proposed a novel pathway for S. cerevisiae that could be applied for producing biodiesel directly. (C) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available