Journal
PROCESS BIOCHEMISTRY
Volume 47, Issue 2, Pages 327-330Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2011.10.036
Keywords
Chitosan oligosaccharides; Histamine; Mast cells; Intracellular Ca2+; Fc epsilon RI
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Funding
- Marine Bioprocess Research Center of the Marine Biotechnology Program
- Ministry of Land, Transport, and Maritime, Republic of Korea
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The activation of mast cells by immunoglobulin E-mediated stimuli is considered as a central event in allergic responses. In this regard, chitosan oligosaccharides (COS) of two different molecular weight ranges (1-3 kDa and 3-5 kDa) were investigated for their capabilities against the activation of RBL-2H3 mast cell sensitized with dinitrophenyl-specific immunoglobulin E antibody and stimulated by antigen dinitrophenyl-bovine serum albumin. It was found that COS significantly inhibited RBL-2H3 cell degranulation via attenuating the releases of histamine and beta-hexosaminidase. Moreover, the inhibitory activity of COS was accompanied by a reduction in intracellular Ca2+ elevation. Notably, the expression of immunoglobulin Fc epsilon receptor I (Fc epsilon RI) in RBL-2H3 cells was down-regulated by COS treatment in a dose-dependent manner. The suppressive effect of COS on RBL-2H3 cell activation suggested that COS may be potential candidates of novel inhibitors against allergic reactions. (C) 2011 Elsevier Ltd. All rights reserved.
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