Journal
PROCESS BIOCHEMISTRY
Volume 45, Issue 6, Pages 973-979Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2010.03.003
Keywords
Erythritol; Osmophilic; Moniliella sp.; Breeding; Mutant; Fermentation
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Funding
- Ministry of Economic Affairs of the Republic of China [89-EC-2A-17-0263]
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An erythritol-producing osmophilic yeast-like fungus, Moniliella sp. 440, was isolated from honey and then successively mutated with iterative rounds of N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment and selection. Six generations of mutants, named N12115-6, N21105-6, N31074-3, N42208-2, N53199-9, and N61188-12, were selected for and produced erythritol at 151.0, 157.2, 177.8, 191.4, 196.6, and 237.8 g/L, respectively, while the wild type strain produced 113.0 g/L erythritol in media containing 40% glucose and 1% yeast extract. The mutant cells were found to have a short rod-like shape, while the wild type cells have a long rod-like shape. The most efficient erythritol producer, N61188-12, assimilated myo-inositol and weakly assimilated erythritol. However, the wild type strain did not assimilate myo-inositol and assimilated erythritol well. In 250-L and 2000-L pilot-scale fermentors, the erythritol production by N61188-12 was 151.4 g/L and 152.4 g/L, respectively. A simple fed-batch culture of strain N61188-12 in a 2000-L fermentor increased erythritol production to 189.4 g/L after 10 days fermentation. (C) 2010 Elsevier Ltd. All rights reserved.
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