4.6 Article

Single cell oil production from hydrolysates of inulin and extract of tubers of Jerusalem artichoke by Rhodotorula mucilaginosa TJY15a

Journal

PROCESS BIOCHEMISTRY
Volume 45, Issue 7, Pages 1121-1126

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2010.04.002

Keywords

Single cell oil; Rhodotorula mucilaginosa; Inulin; Jerusalem artichoke tubers; Fed-batch cultivation

Funding

  1. National Infrastructure of Natural Resources for Science and Technology Program of China [2005DKA21209]
  2. Hi-Tech Research and Development Program of China (863) [2006AA09Z403]

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In this study, we found that Rhodotorula mucilaginosa TJY15a could accumulate 48.8% (w/w) oil from hydrolysate of inulin and its cell dry weight reached 14.8 g/l during the batch cultivation while it could accumulate 48.6% (w/w) oil and 52.2% (w/w) oil from hydrolysate of extract of Jerusalem artichoke tubers and its cell dry weight reached 14.4 g/l and 19.5 g/l during the batch and fed-batch cultivations, respectively. At the end of the fed-batch cultivation, only 0.04% of reducing sugar and 0.08% of total sugar were left in the fermented medium. Over 87.6% of the fatty acids from the yeast strain TJY15a cultivated in the hydrolysate of extract of Jerusalem artichoke tubers was C-16:0, C-18:1 and C-18:2, especially C-18:1 (54.7%). Therefore, the results show that hydrolysates of inulin and extract of Jerusalem artichoke tubers were also the good materials for single cell oil production. (C) 2010 Elsevier Ltd. All rights reserved.

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