Journal
PROCESS BIOCHEMISTRY
Volume 45, Issue 8, Pages 1325-1329Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2010.04.025
Keywords
Fructooligosaccharides; Elution chromatography; Cation exchanger; Poly(styrene-divinyl benzene) resin; Optimization; Process design
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Funding
- Science and Technology Assistance Agency APVV [LPP-0234-06]
- Slovak Grant Agency for Science VEGA [1/0655/09]
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An experimental study aimed at the design and optimization of single-column separation of fructooligosaccharides from mono- and disaccharides was carried out. Amberlite(TM) CR1320Ca, a cation-exchange resin with a calcium ion as the functional group was packed in two laboratory columns of different dimensions. Optimization parameters were the superficial velocity and the column load. In a smaller column, the maximal yield of fructooligosaccharides of 86% and the selectivity of separation of 82% were achieved for the column load of 2.0% and superficial velocity of 5.0 x 10(-5) m s(-1). At the same process conditions in a larger column, the yield and selectivity increased to 90%. (C) 2010 Elsevier Ltd. All rights reserved.
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