Journal
PROCESS BIOCHEMISTRY
Volume 44, Issue 9, Pages 969-974Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2009.04.021
Keywords
Antioxidant; Plant; Extremophil; Psychrophilic enzyme; Photo-oxidation; Oxidative stress
Categories
Funding
- Antarctica Des [S-3607]
- DIUFRO [D109-0081, D108-2002]
- FONDEF [D031-1079]
- CONACyT [82010, 79973, 209658]
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Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O-center dot(2)-) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants. A cDNA Cu/Zn-SOD gene from D. antarctica was cloned into a pET vector and expressed in Escherichia coli BL21-SI. 112 mg/L of recombinant Cu/Zn-SOD was attained in batch cultures in bioreactor. Using Ni-affinity gel chromatography, the recombinant Cu/Zn-SOD was recovered with a purity of 90% and a specific enzyme activity of 749 at 25 degrees C. However, zymogram test showed that the enzyme has more activity at 4 degrees C. This D. antarctica SOD could be used to reduce the oxidation of refrigerated and frozen foods. (C) 2009 Elsevier Ltd. All rights reserved.
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