Journal
PROCESS BIOCHEMISTRY
Volume 44, Issue 8, Pages 854-861Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2009.04.006
Keywords
Chitinase; Chitosanase; Protease; Antioxidant activity; N-Acetyl chitooligosaccharides; Serratia sp.
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Funding
- National Science Council, Taiwan [NSC96-2313-B-032-002-MY3]
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A chitinase (CHT), a chitosanase (CHS) and a protease (PRO) were purified from the culture supernatant of Serratia sp. TKU020 with squid pen as the sole carbon/nitrogen source. The molecular masses of CHT, CHS and PRO determined by SDS-PAGE were approximately 65 kDa, 55 kDa and 55 kDa, respectively. CHT and CHS were inhibited by Mn2+, EDTA and PRO was inhibited by Mg2+, EDTA. The antioxidant activity of TKU020 culture supernatant was 78% (DPPH scavenging ability). N-Acetylglucosamine (GlcNAc) and N-acetyl chitobiose (GlcNAc)(2) were also produced from the culture supernatant by using TKU020 strain fermentation. The maximum production of GlcNAc and (GlcNAc)(2) was 1.3 mg/mL and 2.7 mg/mL, respectively, after 4 days of fermentation. With this method, we have shown that squid pen wastes can be utilized and it is effective in the production of enzymes, antioxidants, and N-acetyl chitooligosaccharides, facilitating its potential use in industrial applications and functional foods. (C) 2009 Elsevier Ltd. All rights reserved.
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