Journal
PROCESS BIOCHEMISTRY
Volume 43, Issue 10, Pages 1019-1032Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2008.06.004
Keywords
enzyme; stability; immobilization; reverse micelle; surfactant coating; chemical modification; CLEAs
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Enzyme stabilization has notable importance due to increasing number of enzyme applications. Stabilization of enzymes in order to realize their full potential as catalysts is discussed in the present review. An overview of the denaturation mechanisms in aqueous and non-aqueous environment is given. Further various methods of enzyme stabilization with respect to their use in aqueous and non-aqueous environment have been given. Using thermophilic enzymes as the reference point, a review of stabilization using various approaches like protein engineering, chemical modifications of enzymes and immobilization has been attempted. Finally, it has been stressed that, for selection of a suitable stabilization approach the intended use and possible interactions between the stabilizer-enzyme have to be taken into consideration. (C) 2008 Elsevier Ltd. All rights reserved.
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