Journal
NATURE COMMUNICATIONS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms9500
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Funding
- NSF as a MRSEC
- Defense Threat Reduction Agency [HDTRA1-13-1-0037]
- National Science Foundation [CBET 1160005, CBET 1264509, CBET 1435957]
- United States Department of Agriculture [NIFA 2014-67021-21585]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1264509, 1160005] Funding Source: National Science Foundation
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Advances in nanotechnology have provided unprecedented physical means to sample molecular space. Living cells provide additional capability in that they identify molecules within complex environments and actuate function. We have merged cells with nanotechnology for an integrated molecular processing network. Here we show that an engineered cell consortium autonomously generates feedback to chemical cues. Moreover, abiotic components are readily assembled onto cells, enabling amplified and 'binned' responses. Specifically, engineered cell populations are triggered by a quorum sensing (QS) signal molecule, autoinducer-2, to express surface-displayed fusions consisting of a fluorescent marker and an affinity peptide. The latter provides means for attaching magnetic nanoparticles to fluorescently activated subpopulations for coalescence into colour-indexed output. The resultant nano-guided cell network assesses QS activity and conveys molecular information as a 'bio-litmus' in a manner read by simple optical means.
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