Epigenetic coordination of embryonic heart transcription by dynamically regulated long noncoding RNAs

The vast majority of mammalian DNA does not encode for proteins but instead is transcribed into noncoding (nc) RNAs having diverse regulatory functions. The poorly characterized subclass of long ncRNAs (IncRNAs) can epigenetically regulate protein-coding genes by interacting locally in cis or distally in trans. A few reports have implicated specific IncRNAs in cardiac development or failure, but precise details of IncRNAs expressed in hearts and how their expression may be altered during embryonic heart development or by adult heart disease is unknown. Using comprehensive quantitative RNA sequencing data from mouse hearts, livers, and skin cells, we identified 321 IncRNAs present in the heart, 117 of which exhibit a cardiac-enriched pattern of expression. By comparing IncRNA profiles of normal embryonic (similar to E14), normal adult, and hypertrophied adult hearts, we defined a distinct fetal IncRNA abundance signature that includes 157 IncRNAs differentially expressed compared with adults (fold-change >= 50%, false discovery rate = 0.02) and that was only poorly recapitulated in hypertrophied hearts (17 differentially expressed IncRNAs; 13 of these observed in embryonic hearts). Analysis of protein-coding mRNAs from the same samples identified 22 concordantly and 11 reciprocally regulated mRNAs within 10 kb of dynamically expressed IncRNAs, and reciprocal relationships of IncRNA and mRNA levels were validated for the Mccc1 and Relb genes using in vitro IncRNA knockdown in C2C12 cells. Network analysis suggested a central role for IncRNAs in modulating NF kappa B-and CREB1-regulated genes during embryonic heart growth and identified multiple mRNAs within these pathways that are also regulated, but independently of IncRNAs.