Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 111, Issue 31, Pages 11497-11502Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1407056111
Keywords
ATP synthase; proton potential; sodium ion potential; bioenergetics
Categories
Funding
- Korea Institute of Ocean Science and Technology [PE99212, PE99263]
- Development of Biohydrogen Production Technology Using the Hyperthermophilic Archaea program of the Ministry of Oceans and Fisheries
- Deutsche Forschungsgemeinschaft [SFB 807, MU801/15-1]
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Thermococcus onnurineus NA1 is known to grow by the anaerobic oxidation of formate to CO2 and H-2, a reaction that operates near thermodynamic equilibrium. Here we demonstrate that this reaction is coupled to ATP synthesis by a transmembrane ion current. Formate oxidation leads to H+ translocation across the cytoplasmic membrane that then drives Na+ translocation. The ion-translocating electron transfer system is rather simple, consisting of only a formate dehydrogenase module, a membrane-bound hydrogenase module, and a multisubunit Na+/H+ antiporter module. The electrochemical Na+ gradient established then drives ATP synthesis. These data give a mechanistic explanation for chemiosmotic energy conservation coupled to formate oxidation to CO2 and H-2. Because it is discussed that the membrane-bound hydrogenase with the Na+/H+ antiporter module are ancestors of complex I of mitochondrial and bacterial electron transport these data also shed light on the evolution of ion transport in complex I-like electron transport chains.
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