Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 111, Issue 13, Pages 4862-4867Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1403059111
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- National Institutes of Health Grant [AI095686]
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Micronemal protein 2 (MIC2) is the key adhesin that supports gliding motility and host cell invasion by Toxoplasma gondii. With a von Willebrand factor A (VWA) domain and six thrombospondin repeat domains (TSR1-6) in its ectodomain, MIC2 connects to the parasite actomyosin system through its cytoplasmic tail. MIC2-associated protein (M2AP) binds noncovalently to the MIC2 ectodomain. MIC2 and M2AP are stored in micronemes as proforms. We find that the MIC2-M2AP ectodomain complex is a highly elongated 1: 1 monomer with M2AP bound to the TSR6 domain. Crystal structures of N-terminal fragments containing the VWA and TSR1 domains for proMIC2 and MIC2 reveal a closed conformation of the VWA domain and how it associates with the TSR1 domain. A long, proline-rich, disulfide-bonded pigtail loop in TSR1 overlaps the VWA domain. Mannose alpha-C-linked to Trp-276 in TSR1 has an unusual C-1(4) chair conformation. The MIC2 VWA domain includes a mobile alpha 5-helix and a 22-residue disordered region containing two disulfide bonds in place of an alpha 6-helix. A hydrophobic residue in the prodomain binds to a pocket adjacent to the alpha 7-helix that pistons in opening of the VWA domain to a putative high-affinity state.
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