Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 111, Issue 30, Pages 10966-10971Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1411802111
Keywords
single-cell method; real-time detection; drug screening
Categories
Funding
- US Air Force Office of Scientific Research Grant [FA9550-09-1-0656]
- T. L. L. Temple Foundation
- John J. and Rebecca Moores Endowment
- State of Texas through the Texas Center for Superconductivity at the University of Houston
Ask authors/readers for more resources
Extraction of intracellular molecules is crucial to the study of cellular signal pathways. Disruption of the cellular membrane remains the established method to release intracellular contents, which inevitably terminates the time course of biological processes. Also, conventional laboratory extractions mostly use bulky materials that ignore the heterogeneity of each cell. In this work, we developed magnetized carbon nanotubes that can be sneaked into and out of cell bodies under a magnetic force. Using a testing model with overexpression of GFP, the nanotubes successfully transported the intracellular GFP out at the single-cell level. The confined nanoscale invasiveness did not change cell viability or proliferation. This study presents the proof of concept of a previously unidentified real-time and single-cell approach to investigate cellular biology, signal messengers, and therapeutic effects with nanomaterials.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available