Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 111, Issue 12, Pages 4495-4500Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1318393111
Keywords
activity; methane cycle; overlooked methane sink
Categories
Funding
- Natural Science Foundation [51108408, 40081198]
- Shanghai Tongji Gao Tingyao Environmental Science and Technology Development Foundation
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The process of nitrite-dependent anaerobic methane oxidation (n-damo) was recently discovered and shown to be mediated by Candidatus Methylomirabilis oxyfera (M. oxyfera). Here, evidence for n-damo in three different freshwater wetlands located in southeastern China was obtained using stable isotope measurements, quantitative PCR assays, and 16S rRNA and particulate methane monooxygenase gene clone library analyses. Stable isotope experiments confirmed the occurrence of n-damo in the examined wetlands, and the potential n-damo rates ranged from 0.31 to 5.43 nmol CO2 per gram of dry soil per day at different depths of soil cores. A combined analysis of 16S rRNA and particulate methane monooxygenase genes demonstrated that M. oxyfera-like bacteria were mainly present in the deep soil with a maximum abundance of 3.2 x 10(7) gene copies per gram of dry soil. It is estimated that similar to 0.51 g of CH4 m(-2) per year could be linked to the n-damo process in the examined wetlands based on the measured potential n-damo rates. This study presents previously unidentified confirmation that the n-damo process is a previously overlooked microbial methane sink in wetlands, and n-damo has the potential to be a globally important methane sink due to increasing nitrogen pollution.
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