Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 111, Issue 10, Pages 3811-3816Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1315359111
Keywords
caveola; signal transduction; heart; plasma membrane
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Japan Association for the Advancement of Medical Equipment
- Takeda Science Foundation
- Mitsubishi Pharma Research Foundation
- Grants-in-Aid for Scientific Research [24390227, 23591088, 24659437] Funding Source: KAKEN
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The actions of catecholamines on adrenergic receptors (ARs) induce sympathetic responses, and sustained activation of the sympathetic nervous system results in disrupted circulatory homeostasis. In cardiomyocytes, alpha 1-ARs localize to flask-shaped membrane microdomains known as caveolae. Caveolae require both caveolin and cavin proteins for their biogenesis and function. However, the functional roles and molecular interactions of caveolar components in cardiomyocytes are poorly understood. Here, we showed that muscle-restricted coiled-coil protein (MURC)/Cavin-4 regulated alpha 1-AR-induced cardiomyocyte hypertrophy through enhancement of ERK1/2 activation in caveolae. MURC/Cavin-4 was expressed in the caveolae and T tubules of cardiomyocytes. MURC/Cavin-4 overexpression distended the caveolae, whereas MURC/Cavin-4 was not essential for their formation. MURC/Cavin-4 deficiency attenuated cardiac hypertrophy induced by alpha 1-AR stimulation in the presence of caveolae. Interestingly, MURC/Cavin-4 bound to alpha 1A-and alpha 1B-ARs as well as ERK1/2 in caveolae, and spatiotemporally modulated MEK/ERK signaling in response to alpha 1-AR stimulation. Thus, MURC/Cavin-4 facilitates ERK1/2 recruitment to caveolae and efficient alpha 1-AR signaling mediated by caveolae in cardiomyocytes, which provides a unique insight into the molecular mechanisms underlying caveola-mediated signaling in cardiac hypertrophy.
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