Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 110, Issue 17, Pages 6795-6799Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1304300110
Keywords
host temperature; irreversible and conformational change; temperature dependence; cryo-EM
Categories
Funding
- National Institutes of Health [R01 AI76331, R01-AI077955]
- Purdue University
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We report on a conformational transition of dengue virus when changing the temperature from that present in its mosquito vectors to that of its human host. Using cryoelectron microscopy, we show that although the virus has a smooth surface, a diameter of similar to 500 angstrom, and little exposed membrane at room temperature, the virions have a bumpy appearance with a diameter of similar to 550 angstrom and some exposed membrane at 37 degrees C. The bumpy structure at 37 degrees C was found to be similar to the previously predicted structure of an intermediate between the smooth mature and fusogenic forms. As humans have a body temperature of 37 degrees C, the bumpy form of the virus would be the form present in humans. Thus, optimal dengue virus vaccines should induce antibodies that preferentially recognize epitopes exposed on the bumpy form of the virus.
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