Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 110, Issue 22, Pages 8790-8794Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1209302110
Keywords
single-cell measurements; MALDI mass spectrometry; baker's yeast
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Funding
- Seventh European Community [PIEF-GA-2011-271895-DiSC-MS]
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Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-D-glucose) and genetically (Delta PFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.
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