Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 110, Issue 11, Pages 4255-4260Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1214046110
Keywords
pregnancy; cancer
Categories
Funding
- Howard Hughes Medical Institute
- National Institutes of Health [HD068546]
- Center for Reproductive Medicine and Infertility
- National Center for Research Resources
- National Center for Advancing Translational Sciences (NCATS), National Institutes of Health [8UL1TR000043]
Ask authors/readers for more resources
We profiled microRNAs (miRNAs) in cell-free serum and plasma samples from human volunteers using deep sequencing of barcoded small RNA cDNA libraries. By introducing calibrator synthetic oligonucleotides during library preparation, we were able to calculate the total as well as specific concentrations of circulating miRNA. Studying trios of samples from newborn babies and their parents we detected placental-specific miRNA in both maternal and newborn circulations and quantitated the relative contribution of placental miRNAs to the circulating pool of miRNAs. Furthermore, sequence variation in the placental miRNA profiles could be traced to the specific placenta of origin. These deep sequencing profiles, which may serve as a model for tumor or disease detection, allow us to define the repertoire of miRNA abundance in the circulation and potential uses as biomarkers.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available