Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 110, Issue 45, Pages 18037-18041Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1314449110
Keywords
cryo-EM; SPEM; 3DEM; EMDB; validation
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Funding
- Medical Research Council [MC_U105184322] Funding Source: researchfish
- Medical Research Council [MC_U105184322] Funding Source: Medline
- MRC [MC_U105184322] Funding Source: UKRI
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Single particle cryo-electron microscopy is currently poised to produce high-resolution structures of many biological assemblies, but several pitfalls can trap the unwary. This critique highlights one problem that is particularly relevant when smaller structures are being studied. It is known as Einstein from noise, in which the experimenter honestly believes they have recorded images of their particles, whereas in reality, most if not all of their data consist of pure noise. Selection of particles using cross-correlation methods can then lead to 3D maps that resemble the model used in the initial selection and provide the illusion of progress. Suggestions are given about how to circumvent the problem.
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