Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 110, Issue 33, Pages 13481-13486Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1304227110
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Funding
- NIGMS Postdoctoral Research Associate (PRAT) Fellowship
- Intramural Research Programs of the National Human Genome Research Institute
- Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics
- estate of Alice Schwarz-Gardos
- estate of John Hunter
- Knell Family
- Peter and Patricia Gruber Award
- National Cancer Institute [R21CA152432]
- National Institutes of Health
- University of Texas MD Anderson Cancer Center Melanoma Specialized Programs of Research Excellence [P50 CA093459]
- Cancer Center Support Grant (CCSG) Core Grant [NCI 5P30 CA006516-46]
- Human Frontier Science Program [RGP0024]
- National Health and Medical Research Council of Australia [1026112, 613686]
- National Cancer Institute
- Eli Lilly and Company
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Synonymous mutations, which do not alter the protein sequence, have been shown to affect protein function [Sauna ZE, Kimchi-Sarfaty C (2011) Nat Rev Genet 12(10):683-691]. However, synonymous mutations are rarely investigated in the cancer genomics field. We used whole-genome and -exome sequencing to identify somatic mutations in 29 melanoma samples. Validation of one synonymous somatic mutation in BCL2L12 in 285 samples identified 12 cases that harbored the recurrent F17F mutation. This mutation led to increased BCL2L12 mRNA and protein levels because of differential targeting of WT and mutant BCL2L12 by hsa-miR-671-5p. Protein made from mutant BCL2L12 transcript bound p53, inhibited UV-induced apoptosis more efficiently than WT BCL2L12, and reduced endogenous p53 target gene transcription. This report shows selection of a recurrent somatic synonymous mutation in cancer. Our data indicate that silent alterations have a role to play in human cancer, emphasizing the importance of their investigation in future cancer genome studies.
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