Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 110, Issue 16, Pages 6459-6464Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1304432110
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Funding
- Chinese National Science Fund for Distinguished Young Scholars [39925023]
- National Natural Science Foundation of China [30530390, 31000408]
- Ministry of Science and Technology of China [2006BAI23B02, 2011BAI15B02]
- Science and Technology Commission of Shanghai municipality [10DZ2251500, 10JC1410300, 11DZ2292400]
- E-Institutes of Shanghai Municipal Education Commission [E03003]
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Retinoic acid inducible gene I (RIG-I) senses viral RNAs and triggers innate antiviral responses through induction of type I IFNs and inflammatory cytokines. However, whether RIG-I interacts with host cellular RNA remains undetermined. Here we report that Rig-I interacts with multiple cellular mRNAs, especially Nf-kappa b1. Rig-I is required for NF-kappa B activity via regulating Nf-kappa b1 expression at posttranscriptional levels. It interacts with the multiple binding sites within 3'-UTR of Nf-kappa b1 mRNA. Further analyses reveal that three distinct tandem motifs enriched in the 3'-UTR fragments can be recognized by Rig-I. The 3'-UTR binding with Rig-I plays a critical role in normal translation of Nf-kappa b1 by recruiting the ribosomal proteins [ribosomal protein L13 (Rpl13) and Rpl8] and rRNAs (18S and 28S). Down-regulation of Rig-I or Rpl13 significantly reduces Nf-kappa b1 and 3'-UTR-mediated luciferase expression levels. These findings indicate that Rig-I functions as a positive regulator for NF-kappa B signaling and is involved in multiple biological processes in addition to host antivirus immunity.
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