Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 110, Issue 5, Pages 1857-1862Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1221840110
Keywords
gene therapy; molecular imaging
Categories
Funding
- California Institute for Regenerative Medicine Training Grant [TG2-01169]
- Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA
- Philip Whitcome training grant
- California Institute for Regenerative Medicine Bridges Training Program
- California Institute for Regenerative Medicine Tools/Technology Award [RT1-01126]
Ask authors/readers for more resources
Positron emission tomography (PET) reporter genes allow noninvasive whole-body imaging of transplanted cells by detection with radiolabeled probes. We used a human deoxycytidine kinase containing three amino acid substitutions within the active site (hdCK3mut) as a reporter gene in combination with the PET probe [F-18]-L-FMAU (1-(2-deoxy-2-(18)fluoro-beta-L-arabinofuranosyl)-5-methyluracil) to monitor models of mouse and human hematopoietic stem cell (HSC) transplantation. These mutations in hdCK3mut expanded the substrate capacity allowing for reporter-specific detection with a thymidine analog probe. Measurements of long-term engrafted cells (up to 32 wk) demonstrated that hdCK3mut expression is maintained in vivo with no counter selection against reporter-labeled cells. Reporter cells retained equivalent engraftment and differentiation capacity being detected in all major hematopoietic lineages and tissues. This reporter gene and probe should be applicable to noninvasively monitor therapeutic cell transplants in multiple tissues.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available